Part:BBa_K4782010

pET 9a low copy plasmid
Name: pET 9a low copy plasmid
Length: 4341 bp

General Properties

T7*Tag sequence and BamH I cloning site are located on the N-terminus of the pET-9a-d(+). Many pET family vectors have their origins in these vectors. Figure 2 depicts the unique sites in the circular map. Because the sequence is numbered according to the pBR322 convention, the T7 expression region on the circular map is inverted. The cloning/expression sequence (Figure 3) of the T7 RNA polymerase-transcribed coding strand is represented by the image below.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 588
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 229
  Illegal NheI site found at 543
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 646
  Illegal BamHI site found at 510
  Illegal XhoI site found at 3552
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 588
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 588
  Illegal NgoMIV site found at 678
  Illegal NgoMIV site found at 1046
  Illegal NgoMIV site found at 1206
  Illegal NgoMIV site found at 1560
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 607
  Illegal SapI site found at 2629
  

Description of Features

Feature	Location	Size	Type
T7 terminator note: transcripton terminator for bacteriophage T7 RNA Polymerase	404...451	48	Terminator
T7 tag product: epitope tag from a t7 major capsid protein translation: MASMTGGQOMG 11 amino acids- 1.1 kDa	519...551	33 bp	CDC
RBS note: ribosome binding site	560...565	33 bp	RBS
T7 promoter note: promoter for bacteriophage T7 RNA polymerase	613...631	19 bp	Promoter
rop gene- rop product - Rop protein translation: VTKOEKTALNMARFIRSOTLTLLEKLNELDADE0ADICESLHDHADELYRSCLARFGDDGENL* 63 amino acids - 7.2 kDa	2192...2383	129 bp	CDS
ori direction : LEFT note: high-copy-number Cole1/pMB1/pBR322/pUC origin of replication	2813...3401	589 bp	rep_origin